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Eto,S.F.; Fernandes,D.C.; Rosolem,M.C.; Marinho-Neto,F.A.; Pizauro,J.M.; Salvador,R.; Moraes,J.R.E.; Moraes,F.R.. |
RESUMO O presente trabalho avaliou o papel do baço no armazenamento e na reativação das linhagens de células B, representadas por células IgM positivas imunomarcadas no tecido esplênico, bem como a funcionalidade dessas células, sobre a cinética dos linfócitos e na produção sistêmica de anticorpos em tilápias-do-nilo (Oreochromis niloticus). Foram separados dois grupos: grupo memória, constituído por peixes previamente imunizados com hemácia de carneiro a 2,5%, para a geração da memória imune, e o grupo naive, que recebeu o mesmo volume de solução salina a 0,65%. Após 32 dias, os dois grupos foram submetidos a uma nova dose do antígeno na mesma concentração, volume e via de inoculação. A reativação dos clones de memória foi evidenciada pelo aumento do... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Teleósteo; Imunologia; Imunoglobulina M; Vacina. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100205 |
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Engrácia Filho,J.R.; Shimada,M.T.; Yunis-Aguinaga,J.; Ramos-Espinoza,F.C.; Moraes,F.R.; Moraes,J.R.E.. |
ABSTRACT Skeletal deformities are frequent problems in cultured fish populations due mainly to unfavorable abiotic conditions, inappropriate nutrition, and genetic factors. These may cause erratic swimming, decrease of conversion rate, growth, and market value. The aim of this case report was to present the occurrence of deformities in juvenile cobia and groupers from an offshore marine farm in Ilhabela, São Paulo, Brazil. Nine cobias and seven juvenile groupers, that presented deformities, were euthanized and fixed in 10% formaldehyde. They were tagged, processed, and stained with "Alcian Blue" (AB) and "Alizarin Red-S" (ARS) for visualization of cartilage and bone tissue, respectively. After evisceration, radiographic examination was performed. The... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Operculum atrophy; Kyphosis; Scoliosis; Saddleback syndrome. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501565 |
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Moraes,J.R.E.; Correia,P.H.A.; Camplesi,A.C.; Moraes,F.R.. |
This study evaluated the use of fibrin glue derived from snake venom in the healing process after canine hysterorrhaphy. Three groups of four animals were submitted to uterine hysterotomy followed by wound closure. In Group 1, double-layer suture was used, the first with Schimieden pattern, the second with Cushing pattern; in Group 2, only fibrin glue; and in Group 3, the same as for Group 1 but with fibrin glue as suture reinforcement. Results indicated that fibrin glue produced less inflammation in the exudative phase, and exacerbated deposition of connective tissue and angiogenesis in the proliferative and maturation phases of the healing process, favoring its evolution. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Healing; Fibrin glue; Uterus; Surgical adhesives; Wound closure. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200003 |
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Garcia,F.; Fujimoto,R.Y.; Martins,M.L.; Moraes,F.R.. |
This work investigated the relationship between water characteristics and infestation of protozoan parasites, Icthyophthirius multifiliis and Trichodina sp., in swordtails (Xiphophorus helleri) and platy (Xiphophorus maculates) collected in an ornamental fish farm in São Paulo, Brazil. Fish were monthly collected from ponds and tanks for one year. The prevalence rates in fish from tanks and ponds were, respectively, 34.2% and 22.5% for I. multifiliis, and 13% and 54.2% for Trichodina sp. Increased electric conductivity and pH provoked reduction of I. multifiliis infestation. Low oxygen concentration increased Trichodina sp. infestation. The use of salt to increase the electric conductivity of water was a method to control I. multifiliis. Low dissolved... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Swordtails; Platy; Parasite; Protozoan; Trichodina sp.; Icthyophthirius multifiliis. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100022 |
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Soares,M.J.V.; Moraes,J.R.E.; Moraes,F.R.. |
IgG and IgM deposits in kidneys of dogs with visceral leishmaniasis (VL) were studied in 25 symptomatic dogs (case) and 15 asymptomatic dogs (control) by an immunohistochemical method. All tested dogs were positive for VL by polymerase chain reaction, enzyme-linked immunosorbent assay, and indirect immunofluorescence test. Kidney fragments were submitted to immunohistochemical reaction. Many morphological patterns of distribution of subendothelial granules were identified for IgG and IgM in glomerular capillaries: global, segmental, diffuse, or focal. Intensity of immunohistochemical reaction to IgG was not significantly different when comparing the symptomatic and the asymptomatic animal groups by Fisher's exact test. IgM reactions were significantly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Leishmania; Kidneys; Glomerulonephritis; Immunoglobulins. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000400004 |
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Fagundes,L.C.; Eto,S.F.; Marcusso,P.F.; Fernandes,D.C.; Marinho- Neto,F.A.; Claudiano,G.S.; Moraes,J.R.E.; Moraes,F.R.; Loyola,W.; Freitas,J.C.; Salvador,R.. |
A bactéria Streptococcus agalactiae é um potente agente causador de surtos por doenças bacterianas em peixes. O estresse provocado pelo manejo zootécnico e pela má qualidade ambiental torna a tilápia susceptível às infecções por essa bactéria. O objetivo do presente trabalho foi avaliar a resistência de tilápias-do-nilo imunizadas com soro hiperimune anti-S. agalactiae, posteriormente desafiadas com cepa homóloga da mesma bactéria. Após determinação da DL 50 de S. agalactiae, 36 tilápias foram distribuídas em quatro aquários, dois para o grupo controle e dois para inoculação celomática para produção de anticorpos anti-S. agalactiae. No 21° e 28° dias, foi coletado sangue para obtenção de soro hiperimune utilizado na transferência passiva. Em seguida, 30... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estreptococose; Imunidade; Peixes; Imunoglobulina; Soro. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200379 |
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